First, let me explain how I came about the creation of my very own, one of a kind, cookie recipe. Oddly enough it all begins with cinnamon rolls. When ever I make homemade cinnamon rolls, I make homemade frosting for them. I take a glob of butter and mix in powdered sugar until it looks right, and then add some milk and vanilla. I generally avoid measuring while cooking. As a scientist most of what I due all day involves measuring. I digress. I like cream cheese frosting better. Same thing but you replace some of the butter with cream cheese. As I was mixing in the powdered sugar, I had a flash of inspiration. When I substitute cream cheese for butter it makes frosting better. mmmmm cheese cake. I put lots of butter in cookies. mmmmm cookies. Hey I could put cream cheese in cookies. So without further introduction I give you my masterpiece.
1 stick butter
1 8oz package of cream cheese
2 eggs
1 1/4 cup sugar
1 1/2 tspn vanilla
Mix
2 1/2 cups flour
1 tspn salt
1/2 tspn baking soda
5 Tblspn Baking coco
mix again
add 3/4 bag of milk chocolate chips (it has been suggested, but not tried, for those who like dark chocolate that use of dark chocolate chip would work quite well)
mix some more
cook 350 for about 12 minutes (preferably on a baking stone)
May this recipe bring you peace, happiness, and . . . something else good.
Thursday, May 21, 2009
Thursday, May 14, 2009
Am I ever going to get out of here?
In science one little mistake can cost you a lot of work.
Case study. Wednesday I sac. 6 mice and take their lymph nodes and spleens. I mush it all up to get the cells out. I kill all the red blood cells, leaving the white blood cells. I then count the cells Not all of them, just enough to let me guess about how many I have. I had about 400 million of the little buggers. Next I stick Antibodies to all the cell I don't want. Stick another antibody to that antibody and then little magnets to that. In Short, I stick little tiny magnets to all the cells I don't want. I then stick the tube in a big magnet, wait a bit, and pour out the cells with no magnets on them. In theory I should a tube full of just the right kind of cells (80-90% CD4+ T cells). Now these cells have had it rough for the past few hour, so I let them rest overnight.
End Day one
After a sound nights sleep, I take the cells stimulate them for 15-45 min. Then I put them on Ice and lyse them (I kill them with soap to get all the innards out) spin out all the junk. Then I measure how much protein is in each of my 10 samples
Takes about half a day but we had lab meeting too
End Day 2
Next day I very carefully measure out each sample so they all have the right amount of protein in them. boil the samples for five min. put them into a gel and seperate the proteins by size. (takes 1.5 hours). I take the gel put on a special nitrocellulose membrane, and transfer the proteins on to the membrane. . . well that was the plan. I put the lid on backwards so the electric current was going the wrong way. Instead of the protein moving onto the membrane from the gel it went the other way, lost and never to be seen again. . .
End Day III
Needless to say I was a little mad.
Case study. Wednesday I sac. 6 mice and take their lymph nodes and spleens. I mush it all up to get the cells out. I kill all the red blood cells, leaving the white blood cells. I then count the cells Not all of them, just enough to let me guess about how many I have. I had about 400 million of the little buggers. Next I stick Antibodies to all the cell I don't want. Stick another antibody to that antibody and then little magnets to that. In Short, I stick little tiny magnets to all the cells I don't want. I then stick the tube in a big magnet, wait a bit, and pour out the cells with no magnets on them. In theory I should a tube full of just the right kind of cells (80-90% CD4+ T cells). Now these cells have had it rough for the past few hour, so I let them rest overnight.
End Day one
After a sound nights sleep, I take the cells stimulate them for 15-45 min. Then I put them on Ice and lyse them (I kill them with soap to get all the innards out) spin out all the junk. Then I measure how much protein is in each of my 10 samples
Takes about half a day but we had lab meeting too
End Day 2
Next day I very carefully measure out each sample so they all have the right amount of protein in them. boil the samples for five min. put them into a gel and seperate the proteins by size. (takes 1.5 hours). I take the gel put on a special nitrocellulose membrane, and transfer the proteins on to the membrane. . . well that was the plan. I put the lid on backwards so the electric current was going the wrong way. Instead of the protein moving onto the membrane from the gel it went the other way, lost and never to be seen again. . .
End Day III
Needless to say I was a little mad.
Tuesday, May 5, 2009
Baby boomers
You hear a lot about generations these days. I think the media likes it as a PC way of categorizing people. Baby boomers, gen X, Y, next, blah blah. Generally it sounds like "kids these days".
Generally I don't like such labels, but I have a bone to pick with one of these groups. Yes I'm talking to you baby boomers. Okay I doubt anyone but my friends and sisters will read this.
As a whole the Baby boomer generation really chaps my hide. This was the group that decided to toss the values of the previous generations. So I get handed a world of drugs, sleaze and collapsed families. Sure some good music came out of it, but that's hardly justification. Let's jump back to when my grandparent grew up. Lower crime, little or no drug use, and lower divorce rate. With the exception of the civil rights movement and technological advances, did the world really move as far forward as they like to boast?
Maybe I'm just bitter? Since, after all these years the baby boomers couldn't even save their own retirement and Social security. And handing my generation their 12 trillion + and growing credit card bill. While creating a college system that puts us 20-30,000$ in debt before we can try to make a living.
Am I being unreasonable?
Generally I don't like such labels, but I have a bone to pick with one of these groups. Yes I'm talking to you baby boomers. Okay I doubt anyone but my friends and sisters will read this.
As a whole the Baby boomer generation really chaps my hide. This was the group that decided to toss the values of the previous generations. So I get handed a world of drugs, sleaze and collapsed families. Sure some good music came out of it, but that's hardly justification. Let's jump back to when my grandparent grew up. Lower crime, little or no drug use, and lower divorce rate. With the exception of the civil rights movement and technological advances, did the world really move as far forward as they like to boast?
Maybe I'm just bitter? Since, after all these years the baby boomers couldn't even save their own retirement and Social security. And handing my generation their 12 trillion + and growing credit card bill. While creating a college system that puts us 20-30,000$ in debt before we can try to make a living.
Am I being unreasonable?
Subscribe to:
Posts (Atom)
.JPG)
